Charles "Charlie" Holloway was an archaeologist who was assigned to assist the USCSS Prometheus expedition in discovering the homeworld of the Engineers on LV-223. He has a romantic relationship with Elizabeth Shaw.
In 2089 during his expedition in the Isle of Skye, Holloway and Shaw discover a star map among several unconnected ancient cultures. They interpret this as an invitation from humanity’s forerunners, or "Engineers". Shaw then contacted Peter Weyland, the elderly CEO of Weyland Corporation for a proposal in order to gain enough funding for an expedition to follow the star map. Weyland agrees with the proposal and funds the creation of the scientific vessel Prometheus to follow the map to the distant moon LV-223.
In 2093, the USCSS Prometheus arrives near the moon LV-223. David, wakes up the crew, including him. As the ship nearly arrives at the planet, Vickers, the misson director briefs the crew. When the ship arrives at LV-223, a team which had him, Elizabeth, David, Fifield, Milburn, and Jackson explore a temple near the ship. Inside, they find a decapitated Engineer, a room filled with urns, and a giant stone head. Janek, the ship's captain tells the crew a storm is coming and the team leaves but, however Fifield and Milburn get lost. In the ship, David and him have a talk. During their talk, David puts some Black Liquid from a urn he took, in Charlie's drink. Meanwhile, Elizabeth and him have intercourse. The next day, the same team goes to the temple to look for Fifield and Milburn. The Black Liquid starts to infect him and the team heads back to the ship. Vickers refuses to let him onboard as she fears his sickness will infect the crew. He says goodbye to Elizabeth and walks to Vickers. He tells her to burn him and she does by burning him to death.