Ford was hired by the Weyland Corporation to partake in an expedition aboard the USCSS Prometheus to the distant moon LV-223. After spending two years in hypersleep, Ford along with the other crew members were awaken. As the Prometheus nearly arrives on LV-223, Vickers, Shaw and Holloway briefed the crew on their mission to find the Engineers. Once the Prometheus lands on LV-223, the team (consisting of Shaw, Holloway, David, Millburn, Jackson, Fifield and Ford herself) explores a large artificial structure nearby. While inside the temple, the team came across a water-related terraforming system that made the air within the temple breathable. As the team made their made it deeper into the temple, David activates the temple's hologram, which led them to the preserved remains of an Engineer. Due to the team's tampering, the temple's interior environment begins change drastically, which forced Ford and Shaw to isolate the head of the Engineer's remains for research. While the team was on its way back to the Prometheus due to the approaching windstorm, Ford accidentally dropped the Engineer's head, forcing Shaw to save it in the midst of the windstorm.
Ford later assisted Shaw in performing research on the Engineer's head. Ford used a synapse reestablisher to awaken dormant neurons in the brain tissue in order to learn more about it. While it succeeded at first, it backfires shortly after and causes the Engineer's head to explode. After Shaw was infected by a creature during her intercourse with Holloway (who was infected by the black liquid), Ford was ordered to prepare her for hypersleep. Ford tried to awaken unconscious Shaw by slapping her cheeks repeatedly, only to be knocked out by Shaw by surprise. Ford later escorted Peter Weyland and his team to meet the Last Engineer within the temple. The Engineer reacted hostilely towards the team and begins to kill them one by one. She becomes frozen by fear and is hit hard in her chest by the Engineer and sent flying against the floor, killing her instantly.