The Second Acheron Queen was the second Xenomorph Queen that inhabited Acheron (LV-426) that was encountered by a unit of Colonial Marines from the USS Sephora. She was bred by Weyland-Yutani through the use of one of the Xenomorph eggs found aboard the Derelict spacecraft. She was apparently killed when she was thrown out of a starship on the verge of breaching LV-426's atmosphere by Captain Jeremy Cruz.
Following the death of Ellen Ripley on Fiorina 161, Michael Weyland traveled to LV-426 in hopes of finding any remaining Xenomorph specimens. His search was unsuccessful as all of the Xenomorphs perished during the atmosphere processor's meltdown. Michael was eventually able to locate the Derelict on LV-426 and constructed the Origin Facility around the vessel's remains, where Weyland-Yutani used the eggs found aboard the spacecraft to breed their own Xenomorph population. The Queen was housed in a large chamber within the research complex for study.
When facility was infiltrated by Corporal Winter and Private O'Neal from the USS Sephora, the Marines deactivated numerous security systems across the base during their assault, allowing the Queen to break free of her restraints. Despite attempts by Weyland-Yutani scientists to subdue the Queen with gas and neurotoxins, she was able to escape the chamber in which she was being held, slaughtering several scientists in the process.
Rampaging through the base, the Queen later encountered Winter and O'Neal again when the Marines responded to a downed USCM dropship and killed its pilot Ruiz. Unable to fight the Queen with the weapons available to them, the Marines were forced to run and hide until a second dropship, piloted by Lieutenant Reid, arrived to provide air support; although Reid failed to hit the Queen, the weapons fire drove her off.
The Queen later managed to board a Weyland-Yutani FTL-capable starship on the planet before it departed, once again encountering the Sephora Marines when they crashed into the vessel's cargo hold in their dropship. After stalking Winter through the hold, the Queen was ejected through the open cargo doors on a delivery catapult, although she managed to cling to the outside of the vessel and even began climbing back aboard. In a desperate move, Captain Cruz, wounded and trapped inside the wreckage of the crashed dropship, activated the craft's engines, slamming into the Queen and sending both tumbling out of the spaceship as it prepared to breach LV-426's atmosphere, sending them both falling to their demise.